Feeding technology

The Kuhner Feeding Technology allows reliable screening on a microliter scale to ensure optimum strain selection. 

The Kuhner Feeding Technology provides an innovative tool for efficient high-throughput screenings in fed-batch mode. The diffusion-driven fed-batch system is delivered sterile and ready-to-use for instant application in the lab. The Kuhner Shaker GmbH offers three items applying the Kuhner Feeding Technology: Feed Plate®, FeedBeads® and FeedTubes®. 

By using the fed-batch mode instead of batch cultivations, overflow metabolism with undesired side-product formation can be significantly reduced and oxygen limited conditions can be avoided. Thereby, screening results are improved, and a higher product formation is achieved. 

Using the Kuhner Feeding Technology, cultivation conditions of the strain screenings are more comparable to those on a production scale, since overflow metabolism, pH inhibition, and oxygen limitation are prevented. Thus, an unfavorable strain selection is avoided already early on. Comparable and reproducible conditions in screening allow you to identify the optimal production strain already at an early point. Consequently, this reduces costs as well as time-consuming process adaptations during later stages in process development. 

Benefit: 

• Universal tool for MTP, shake QWflasks and tubes 
• Various substrates and release rates for individual needs 
• Fast and easily integrated sterile and ready-to-use 
• Reducing negative influences O2-limitation, by-products and overflow, pH-shifts 
• Improving Screening controlled cell metabolism and higher significance of results 
• Simplified scale-up starting process development one step earlier 
• Selection of the best strain for later production process 
• High product yields improved cell metabolism extended cultivation time 
• Reduction of costs avoidance of unforeseen development costs